Periódicos Brasileiros em Medicina Veterinária e Zootecnia

p. 1273-1280

Screening Jatropha genotypes for aluminum tolerance using the solution-paper method

Domiciano Silva Rosado, RenatoSessa Fialho, GustavoAnair Souto Dias, BrunaBarbosa Rosado, TatianaEmilia Prieto Martinez, HerminiaGalvêas Laviola, Bruno

The identification of genetic material tolerant to aluminum (Al) can offer important advantages to breeding programs and to recommendations for cultivars with the aim of utilizing acidic soil. Based on this, our aim was to determine, by using the solution-paper method, the aluminum concentration that best demonstrates tolerance of Jatropha accessions to this element. For this study, seeds from eight Jatropha accessions (CNPAE 114, CNPAE 154, CNPAE 169, CNPAE 178, CNPAE 218, CNPAE 200, CNPAE 226, CNPAE 228) were placed in a germination chamber with different concentrations of Al (0, 85, 155, 225 and 295 mg L-1) using the solution-paper technique. After 14 days, the length of the primary root was evaluated and the percentage by which those subjected to doses of Al was calculated. A concentration of 85 mg L-1 was the level that separated the accessions in terms of Al tolerance; the accessions CNPAE 114 and CNPAE 154 were the most sensitive to the presence of this toxic element in the solution. It was concluded that the solution-paper method is efficient and can be used in Jatropha breeding programs for the selection of Al-tolerant genotypes.

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