Trinta anos de tecnologia de reprodução assistida no gato doméstico: Um resumo selecionado
Pope, C. Earle
The first kittens to be born after embryo transfer (ET) in the cat (Schriver and Kraemer, 1978) were reported four decades ago. The births of kittens after in vitro fertilization (IVF)/ET (Goodrowe et al., 1988) and after embryo cryopreservation/ET (Dresser et al., 1988) were described 10 years later. In an early report it was established that embryo donors had the ability ot exhibit repeated ovarian stimulatory response to multiple gonadotropin treatments (porcine FSH; Dresser et al., 1987). After studies on gonadotropin-induced hyperstimulation of follicular development for recovery and ET of fresh and cryopreserved in vivo derived uterine stage embryos from mated donors (Dresser et al., 1988; Pope et al., 1989), we transitioned into developing methods for in vitro production of domestic cat embryos for the purpose of applying the technology to support conservation of threatened and endangered felid species (Pope et al., 1993). Since then, techniques for the in vitro production of cat embryos have been developed sufficiently to allow births of kittens after transfer of embryos derived by an assortment of in vitro techniques, including cryopreservation (Pope et al., 1994; Gómez et al., 2003a; Pope et al., 2012a, b; Galiguis et al., 2014), intracytoplasmic sperm injection (ICSI; Pope et al., 1998; Gómez et al., 2000; Pope et al., 2012a), gender selection (Pope et al., 2009) and somatic cell nuclear transfer (SCNT) (Gómez et al., 2004; 2008; 2009).(AU)
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