Maturação de oócitos bovinos em meio suplementado com captopril
Rodrigues, Viviany de SousaBranco, Yndyra Nayan Teixeira Carvalho CasteloSilva, Jefferson Hallisson Lustosa daPrado, Bruno da SilvaBranco, Marlon de Araújo CasteloSousa Júnior, Antônio deCarvalho, Geraldo Magela CôrtesSouza, José Adalmir Torres de
The aim of this research was to evaluate the effects of supplementation of maturation medium withcaptopril in oocyte in vitro maturation (IVM). 470 CCOs were recovered and classified into grades I and II,being divided into four groups: G1 (n = 56), that was control group; G2 (n = 152) 20 mM of captopril; G3 (n =126) 40 uM of captopril; and G4 (n = 136) 80 uM of captopril, then these were subsequently submitted to IVMprocess for 24 hours at 38.5°C in 5% CO2. After 24 hours of maturation, oocytes were denuded and evaluatedfor the first polar body extrusion and were, therefore, considered matured. The addition of the Captopril inmedium of oocytes maturation has not improved the IVM rate.(AU)
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