Avaliação do desenvolvimento in vitro de embriões bovinos após exposição à crotamina linear

Silva, Mirelly Mirna Alves de SousaCampelo, Iana SalesCavalcanti, Camila MunizMelo, Luciana MagalhãesBaptista, Gandhi RádisTeixeira, Dárcio Ítalo AlvesFreitas, Vicente José de Figueirêdo

The present study investigated the effects of different concentrations of linear crotamine (CrL), used asagent for cell transfection, on in vitro development of bovine embryos. Embryos were exposed to 0; 5 and 12.5µM of CrL in Synthetic Oviductal Fluid (SOF) for 6 h. Vehicle solution was NaCl 150 mM and not exposedembryos (in vitro fertilized-IVF group) was included. In vitro developmental competence was evaluated bycleavage (day 2), blastocyst (day 7 and 8), and hatching (day 8) rates. No difference (P > 0.05) was observedamong CrL groups and control group (IVF) for all observed parameters. In conclusion, the use of the tested CrLconcentrations for 6 h during in vitro culture of bovine embryos have no deleterious effects on embryodevelopment.(AU)

1. embriotoxicidade
2. cell-penetration peptide
3. linear crotamine
4. crotamina linear
5. peptídeo de penetração celular
6. embryotoxicity