Avaliação da temperatura de transporte de folículos ovarianos bovinos coletados nos períodos chuvoso e seco da região norte do Tocantins
Custódio, Ana Caroline OngarattoSant, Carolina Luciano DalSouza, Isadora Afonso deGonçalves, Victor MoreiraNunes, Mariana Coelho PinheiroLima, Ana Kelen Felipe
The objective of this study was to evaluate the effect of temperature and transport time on the CumulusComplex - oophorus (COC). We evaluated 181 cattle COCs obtained from 15 pairs of ovaries, and 743 ovarianfollicles obtained from 30 pairs of ovaries subjected to histological processing. They evaluated the transport at4°C and 37°C in the period of 6:18 hours. After aspiration, the COCs obtained were stained with Trypan Blue toverify the integrity of the cell membrane and / or oocyte, and made the counting of follicles. The ovariestransported at lower temperatures and in shorter time showed better viability, suggesting maintaining theintegrity of the plasma membrane of cells COCs. As the temperature rises, the viability of COCs decreases as thenumber of follicles, suggesting damage to the membranes of such cells. Thus, it was concluded that COCs andovarian follicles undergo with temperature and transport time.(AU)
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