Avaliação da integridade da membrana plasmática e do potencial de atividade mitocondrial de sêmen criopreservado de touros frente a ação do (Limoneno (R)-(+)
Branco, Yndyra Nayan Teixeira Carvalho CasteloBranco, Marlon de Araújo CasteloSousa Junior, Antonio deRodrigues, Viviany de SousaMoraes Junior, Felipe de JesusEvangelista, Luanna Soares de MeloNascimento, Isolda Márcia Rocha doSouza, José Adalmir Torres de
This study was conducted to evaluate the effect of different concentrations of limonene (R)-(+) (50 µM,100 µM and 150 µM) in supplementing the diluter of bulls freezing semen. Thirty-six ejaculated from fourCurraleiro-Pé-Duro bulls were used for cryopreservation. The epifluorescence microscopy was used todetermine the plasmatic integrity and mitochondrial activity potential. No was observed effect on the integrity ofplasma membrane nor mitochondrial activity potential when cryopreserved by adding limonene R - (+). Theresults obtained in this study allow to conclude that supplementation limonene (R) - (+) on diluter of bullsfreezing semen does not interfere with the integrity of the plasma membrane or the potential of spermmitochondrial activity.(AU)
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