Angiotensina-(1-7): Efeito na maturação in vitro de oócitos ovinos

Costa, Andréia da SilvaCarvalho, Muriel AlvesCosta, Amilton Paulo RaposoBranco, Yndyra Nayan Teixeira Carvalho CasteloLeite, Marina CarvalhoCosta, Terysdalva Pereira daSilva, Yanne Aciole daFeitosa, Lauro César Soares

Aimed to evaluate the effect of addition of angiotensin-(1-7) in the middle of in vitro maturation ofsheep oocytes. Seventy-four oocytes of sheep ovaries slaughtered in fridg were matured in vitro: I - control(n=22), II - Ang-(1-7) at 1 uM (n=24), III - Ang-(1-7) at 1 uM+A-779 at 1uM (n=28) in microdropletscontaining 100 µL of maturation medium plus 10% fetal bovine serum under mineral oil in the incubatoratmosphere 5% CO2 in air at 38.5°C for a period of 18 hours. After this period, the oocytes were denuded andobserved the extrusion of the first polar body. Statistical analysis was performed using the t test at 5%probability. The addition of angiotensin-(1-7) at 1 uM, as well as the addition of angiotensin-(1-7) at 1 uMtogether with its specific antagonist A-779 at the same concentration, decrease the in vitro maturation rate ovine oocytes.(AU)

1. reproductive bio techniques
2. biotécnicas reprodutivas
3. in vitro production
4. produção in vitro
5. pequenos ruminantes
6. small ruminants