Adição do Ácido Fólico na criopreservação de sêmen ovino da raça Santa Inês
Barros, Filipe NunesSá, Priscilla Niely Costa deBarçante, Felipe Pereira da SilvaBranco, Marlon de Araújo CasteloSousa Filho, Marco Antônio Celestino deBarros, Dayse AndradeLopes Neto, Bruno de OliveiraSouza, José Adalmir Torres de
We evaluated the addition of folic acid to the extender TRIS-egg yolk used in cryopreservation, seekingimprovements for the sperm kinetics and mitochondrial activity post-thawing. Were evaluated sevenejaculates/animal from six Santa Inês by Artificial Vagina, with minimum values of motility by 70.0% and vigor3. We evaluated the seminal pool for concentration and morphology. We diluted in Tris-egg yolk extender forcryopreservation, dividing it in control, Group 2 we added 10.000 uM and Group 3 we added 5000 uM of folicacid. We froze in straws(0.25 mL). Was evaluated for motility and vigor, post-thawing by slow term-resistancetest, the times T0, T60, T120 and T180 minutes one week after. For the mitochondrial activity evaluation, wasused cationic lipophilic fluorochrome JC-1. The folic acid effects were analyzed using ANOVA followed byStudent Newman-Keuls test. The groups with folic acid showed significant higher motility. Mitochondrialactivity did not differ between groups.(AU)
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