Cryopreservação de semen: desafios e perspectivas
Squires, E. L
Frozen semen has many advantages over fresh or cooled semen .Management of the stallion before and during the freezing process is extremely important for obtaining good quality frozen/thawed semen.Frequency of ejaculation will affect the quality of frozen semen as well as age of the stallion and breed.Futhermore the initial concentration prior to freezing also affects the post-thaw motility.Other factors affecting freezability include type of cryoprotectant and level of glycerol.Cooling rate from minus 10 to minus 50 degree centrigrade do not appear to affect post thaw motility but the concentration within a 0.5 ml straw does affect motility. Proper handling and storage of frozen semen is extremenly important and stallion semen can last indefinitly if manintained in liquid nitrogen(AU)
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