Molecular cloning of a hyaluronidase from Bothrops pauloensis venom gland

Castanheira, Letícia EufálioRodrigues, Renata SantosFrança, Johara BoldriniFonseca, Fernanda P PHenrique-Silva, FlávioBrandeburgo, Maria I HomsiRodrigues, Veridiana M

Hyaluronate is one of the major components of extracellular matrix from vertebrates whose breakdown is catalyzed by the enzyme hyaluronidase. These enzymes are widely described in snake venoms, in which they facilitate the spreading of the main toxins in the victim’s body during the envenoming. Snake venoms also present some variants (hyaluronidases-like substances) that are probably originated by alternative splicing, even though their relevance in envenomation is still under investigation. Hyaluronidases-like proteins have not yet been purified from any snake venom, but the cDNA that encodes these toxins was already identified in snake venom glands by transcriptomic analysis. Herein, we report the cloning and in silico analysis of the first hyaluronidase-like proteins from a Brazilian snake venom.(AU)

1. Alternative splicing
2. Snake venom
3. Hyaluronidase-like