Periódicos Brasileiros em Medicina Veterinária e Zootecnia

Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene

Carnieli JR, P.M. Ventura, A.L. Durigon, E.

A plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested. The presence of the pSH-G plasmid was confirmed by means of polymerase chain reaction (PCR) after each of ten cell passages, and the results were positive. The stable BHK-21/pSH-G+ clone obtained can be used in the study of rabies as well as in the production of vaccines.

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