Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene
Carnieli JR, P.M. Ventura, A.L. Durigon, E.
A plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested. The presence of the pSH-G plasmid was confirmed by means of polymerase chain reaction (PCR) after each of ten cell passages, and the results were positive. The stable BHK-21/pSH-G+ clone obtained can be used in the study of rabies as well as in the production of vaccines.Texto completo