Periódicos Brasileiros em Medicina Veterinária e Zootecnia

p. 229-233

Influência da idade sobre a qualidade de oócitos imaturos caninos

TEIXEIRA, Germano GonçalvesPINHEIRO, Evelyn de CastroTEIXEIRA, Dárcio Ítalo AlvesBERSANO, Paulo Ricardo de OliveiraPEREIRA, Leda Maria Costa

Among some factors that influence the quality of canine oocytes, age is well studied. Knowing that the morphological assessment of oocytes is a useful tool for this analysis, the use of new techniques such as the brilliant cresyl blue staining (BCB) increases this assessment. The objective of this work was to analyze the quantity and the oocyte quality of bitches in different age groups through the conventional morphological evaluation, in addition to the bright cresyl blue dye. For this purpose, 2.302 oocytes from 33 bitches were divided into four age groups from 1 to 3 years old; 4-6, 7-10 and over 10 years old and their oocytes were classified as G1, G2 and G3, as well as stained (BCB (+)) and non-stained (BCB (-)). The values were compared using the Kruskal test -Wallis and Dunn post-test, by which it was possible to obtain higher amounts of G1 and BCB (+) oocytes in the range of 1 to3 years, indicating that this range oocytes have a greater chance of success in fertilization processes and in vitro production, for example.(AU)

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