Efeito da adição de nanopartículas de Óxido de Zinco ao diluente de congelação do sêmen ovino

TOBAL, Lucas Facundo MouraARRUDA, Lúcia Cristina PereiraOLIVEIRA, Aline Saraiva deCARNEIRO, Gustavo FerrerGUERRA, Maria Madalena Pessoa

The aim of this study was to evaluate the effect of adding zinc oxide nanoparticles to the ram semen cryopreservation diluter. Semen pools (n=6), from three Santa Inês breeders, were diluted in Tris-yolk (5% glycerol), supplemented with zinc oxide nanoparticles (0, 25, 75 and 150μg/mL) at a concentration of 200x10 6 sperm/mL. The samples were frozen in an automated system and stored in liquid nitrogen (-196 °C). At the timeof analysis, the semen samples were thawed (37 °C/30s) and evaluated for sperm kinetics, plasma and acrosomal membrane integrity and mitochondrial membrane potential. There was no significant difference (p>0.05) between the experimental groups in the parameters of kinetics, plasma membrane and acrosome integrity. As for the potential of mitochondrial membrane, all treated groups were significantly (p≤0.05) larger than the control. It was concluded that the zinc oxide nanoparticles increase the mitochondrial membrane potential of ram sperm submitted to the freezing/thawing process.(AU)

1. Nanotechnology
2. cryopreservation
3. ram