O uso de RFLP na filogeografia de Carollia perspicillata, Linnaeus 1758 (Chiroptera: Phyllostomidae).
an, Anan, Anan, AnaDavid Ditchfield, Albert
Molecular data has been used succesfully in evolutionary biology, allowing accurate estimates forgenetic diversity within and between populations in a species, identify clades and test biogeographic hypothesis.The short-tailed fruit bat Carollia perspicillata belongs to the subfamily Carollinae (Chiroptera: Phyllostomidae)and is distributed from Mexico to Brazil, Paraguay and Argentina. In a previous study using mtDNA cytochromeb gene sequences, two haplotype groups were identified in Latin America for C. perspicillata. One of these groups, identified as Clade B, was distributed along the Brazlian Atlantic Forest, while the other group, Clade A,occurred from Central America and the Guyanas to Northeastern Brazil, where it co-occurred in full sympatry forsome locaties in the Northern portion of the Atlantic Forest. The objective of this study was to determine if RFLPidentifies the same clades previously identified from cytochrome b sequences, and if the individuals from theAmazon region, which had not been sampled in the previous study, would fit within one of these clades. Theresults show that Amazonian samples belong to both clades and suggests that the Amazon region might be agradient or cline with a transition from Clade A (Northern) and Clade B (Coastal and Southern), that developedperhaps due to a break in gene flow due to geographic isolation.