Periódicos Brasileiros em Medicina Veterinária e Zootecnia

p. 1265-1268

Production of Brazilian human norovirus VLPs and comparison of purification methods

Lamounier, Thais Alves da CostaOliveira, Layssa Miranda deCamargo, Brenda Rabello deRodrigues, Kelly BarretoNoronha, Eliane FerreiraRibeiro, Bergmann MoraisNagata, Tatsuya

Abstract Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.(AU)

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