Detection of both vanA & vanBgenes in vanA phenotypes of Enterococci by Taq Man RT-PCR

Mirzaei, BahmanBabaei, RyhaneAsiabar, Akbar Pour DadashBameri, Zakaria

Twenty seven isolates of vancomycin resistant Enterococci based on the disk diffusion and E- test have been screened; being found eight (0.3%) clinical isolates of vanA & vanB through Taq Man Real Time PCR assay. This study shows the presence of both vanA & vanB genotypes in vanA phenotypes clinical isolates in the three hospitals in Iran.

1. vanA & vanB genotypes
2. vancomycin
3. Enterococci
4. Taq Man Real Time PCR