Evaluation of tests to predict metallo-beta-lactamase in cystic fibrosis (CF) and non-(CF) Pseudomonas

Perez, Leandro Reus RodriguesLimberger, Mariana FagundesCosti, RicardoDias, Cícero Armídio GomesBarth, Afonso Luís

Double disks synergy test (DDST) and combined disks test (CD) were evaluated to predict the presence of metallo-β-lactamase in 70 Pseudomonas aeruginosa isolates recovered from cystic fibrosis and non-cystic fibrosis patients. DDST CAZ-EDTA 1 cm and CD IMP-EDTA tests showed the best accuracy (94.3%). Furthermore, for other combinations, accuracy unsatisfactory was obtained.

1. Phenotypic tests
2. Pseudomonas aeruginosa
3. Metallo-β-lactamase