Genotypic and phenotypic detection of efflux pump in Rhodococcus equi

Gressler, Letícia TrevisanVargas, Agueda Castagna deCosta, Mateus Matiuzzi daPõtter, LucianaSilveira, Bibiana Petri daSangioni, Luis AntônioBotton, Sônia de Avila

The req_39680 gene, associated to a putative efflux system, was detected in 60% (54/90) of R. equi isolates by PCR. The phenotypic expression of efflux mechanism was verified in 20% of the isolates using ethidium bromide. For the first time, the expression of efflux mechanism was demonstrated in R. equi.

1. Ethidium bromide
2. Efflux pump
3. Rhodococcus equi
4. VapA
5. Multidrug resistance