Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

Leal, C A GCarvalho-Castro, G ACottorello, A CLeite, R CFigueiredo, H C P

The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.(AU)

1. Clinical samples
2. WSSV
3. Sensitivity
4. Real-time PCR
5. WSD