Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis

Santos, Elisane Odriosolla dosMichelon, MarianoFurlong, Eliana BadialeBurkert, Janaína Fernandes de MedeirosKalil, Susana JulianoBurkert, Carlos André Veiga

The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass and protein content was observed according to the 2v5-1 fractional design. These factors were further evaluated using a central composite design and response surface methodology, and an empirical model for protein content was established and validated. The biomass of Yarrowia lipolytica NRRL YB-423 reached 19.5 ± 1.0 g/L in shaken flasks cultivation, with a protein content of 20.1 ± 0.6% (w/w).

1. yeast biomass
2. Yarrowia lipolytica
3. single cell protein
4. Raw glycerol