Periódicos Brasileiros em Medicina Veterinária e Zootecnia

A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel

Qiu, ShanlianChen, JichenLin, SiLin, Xinjian

Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.

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