Detection of Bartonella henselae in defibrinated sheep blood used for culture media supplementation

Drummond, Marina RovaniPitassi, Luiza H. U.Lania, Bruno G.Santos, Silvio R. C. dosGilioli, RovilsonVelho, Paulo E. N. F.

Bartonella henselae was detected in defibrinated sheep blood employed in supplementing a selective bacteria culture medium by nested PCR. We recommended that highly sensitive technical tests be run to ensure a sterile culture medium for Bartonella spp. isolation, since infected blood samples used in preparation could lead to false-positive results.

1. Culture Media
2. False Positive Reactions
3. Bartonella
4. Sheep-Blood Contamination
5. Polymerase Chain Reaction