Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping

Miyata, MarceloSantos, Adolfo Carlos BarretoMendes, Natália HelenaCunha, Eunice AtsukoMelo, Fernando Augusto Fiúza deLeite, Clarice Queico Fujimura

We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.

1. DNA extraction
2. Mycobacterium tuberculosis
3. thermolysis
4. CTAB