Enumeration of heterotrophic bacteria in water for dialysis: comparison of the efficiency of Reasoner'2 agar and plate count agar

Bugno, AdrianaAparecida Buzzo Almodóvar, AdrianaCaldas Pereira, Tatiana

Due to the fact the incubation conditions may influence the microbiological evaluation of water for dialysis, the objective of the present study was the comparison of the efficiency of R2A and PCA media in the enumeration of heterotrophic bacteria in 193 samples of water collected in dialysis clinics from 12 cities in São Paulo, between October and December 2007. Results showed counts significantly greater in R2A, suggesting that enumeration should be carried out in R2A, suggesting that enumeration should be carried out in R2A agar associated with longer incubation times, because of the greater sensitivity.

1. Reasoner'2 Agar
2. Plate Count Agar
3. Water for dialysis
4. pour plate
5. heterotrophic bacteria