Experimental model of Candida Albicans (serotypes A and B) adherence In Vitro

Pires, Maria de Fátima CostaCorrêa, BeneditoGambale, WalderezPaula, Claudete Rodrigues

The adherence of Candida albicans is considered an important virulence factor since it determines the colonization of the yeast in various regions of the host, being the initial cause of the infection. In this work we studied the adherence of C. albicans (serotypes A and B) to HeLa and Vero cells after culture for 18 h and 30 h in chemically defined medium (YNB) plus carbohydrate sources such as glucose, sucrose, maltose, galactose and mannose (500 mM). Each assay was performed in triplicate. The best results were obtained with the medium containing glucose. The adherence was greatest in 18 h cultures at 37§C and at 1 h contact time. The interaction of yeast cells with the host cells was studied by scanning microscopy.