Duguetia furfuracea (A. St.-Hil.) Benth. & Hook. f.: microscopia e variação sazonal na composição química do óleo essencial e atividade antioxidante

Rhoden, S. L.Faoro, J. A. M.Oliveira Junior, P. C.Cardoso, C. A. L.Mussury, R. M.Machado, C. D.Manfron, J.Souza, M. F.Souza, N. L. B.Formagio, A. S. N.

Abstract Duguetia furfuracea (A. St.-Hil.) Benth. & Hook. f., Annonaceae, occurs in the state of Mato Grosso do Sul, and its leaves are a source of essential oil that can be obtained by hydrodistillation. However, there is no scientific evidence demonstrating seasonal variation in the chemical composition of this species. This study evaluated the chemical composition of the essential oil of D. furfuracea (EODF) collected seasonally over 12 months, as well as the microscopy and histochemical analysis from leaves and stems, and antioxidant activity. The essential oil was obtained from the leaves by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS). The analysis of EODF identified 24 compounds, predominantly monoterpenes (46.91% - 17.03%) and sesquiterpenes (42.51% - 17.82%), with β-pinene being the major compound (31.06%). Precipitation did not influence the yield of essential oil from D. furfuracea. Essential oil cells were predominantly found in the palisade parenchyma layers, especially below the leaf surface, mainly on the adaxial part of the leaves. The antioxidant activity of the essential oil (EODF-JL) can be partially attributed to the presence of the major components sabinene and γ-terpinene, which are found in higher amounts exclusively in this sample. The study highlighted that seasonal variation could influence the chemical composition of the essential oil of D. furfuracea and its antioxidant activity. Additionally, microscopy and histochemical information provided insights for the identification and quality control of this species.