Periódicos Brasileiros em Medicina Veterinária e Zootecnia

p. 710-714

Padronizacão da técnica de imunoistoquímica para o diagnóstico etiológico de rotina da diarréia bovina a vírus

Andrade, G. ISerra, C. VBarbosa-Stancioli, E. FSerakides, RLobato, Z. I. P

The immunohistochemistry standardization for bovine viral diarrhea virus (BVDV) diagnostic was described. The formalin-fixed tissue samples from a heifer with mucosal disease were used as positive control. The validation of the first phase results was performed using samples from an aborted fetus and a calf infected with reference strains of BVDV. The best results were seen using monoclonal antibodies and a commercial kit consisting of labelled streptavidin biotin (LSAB) reagents and the diaminobenzidine (DAB) substrate-chromogen reagent. The immunohistochemistry demonstrated to be an useful method for routine diagnosis for the controll and detection of BVDV infection.(AU)

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