Periódicos Brasileiros em Medicina Veterinária e Zootecnia

p. 61-66

Energy - restricted diet during lactation programs ovarian growth factor and gonadotrop in receptor expression in rats

Ferreira, R VGombar, F MSampaio, F JRamos, C F

The goal of this study was to evaluate whether a maternal energy - restricted diet could program ovarian angiogenesis and growth factor and gonadotropin receptors expression in rats . After parturition, s ix Wistar rats with 6 pups each were random ly assigned to one of the following groups: control group (C), with free access to a standard laboratory diet containing 23% protein; and energy - restricted gr oup (ER), receiving 60% of the amount consumed by the control group. After weaning, all female pup s had free access to the standard laboratory diet until 90 days of age, when they were sacrificed during the proestru s stage . Blood was collected for further evaluation of serum estradiol levels . One ovary was stored at - 80ºC for RT - PCR analysis while the other one was paraffin embedded, sectioned at 5 - μm thickness and processed by routine histological analys i s for evaluation of vessel density . The ER group had a reduction in follicle stimulating hormone (C = 2.36 ± 0.21, ER = 0.97 ± 0.15, P < 0.001) and lu teinizing hormone ( C = 1.81 ± 0.11, ER = 0.74 ± 0.11, P < 0.0004) receptor expression . This group also had an increase in gene expression of basic fibroblast growth (C = 0.51 ± 0.07, ER = 0.72 ± 0.06 , P < 0.04) and vascular endothelial growth factors (C = 0.82 ± 0.03, ER = 1.06 ± 0.08, P < 0.02). E stradiol serum concentration, vessel density, kinase - insert domain receptor, Fms - like tyrosine kinase - 1 and fibroblast growth factor receptor - 1 gene expressions were unchanged. The results indicated that a materna l energy - restricted diet during lactation programs the ovarian response to FSH, LH and angiogenic factors.(AU)

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