Bovine serum albumin improves in vitro development of caprine preantral follicles
Rodrigues, G QSilva, C M GFaustino, L RBruno, J BMagalhães, D MCampello, C CFigueiredo, J R
This work investigated the effects of different protein supplements (fetal calf serum (FCS) and bovine serum albumin (BSA)) on the in vitro development of caprine preantral follicles. Preantral follicles (> 150 um) were isolated from ovarian cortex fragments and individually cultivated in a-MFM medium in an incubator at 37ºC for 24 days with 5% atmospheric Co2, and supplemented with either BSA at 1,25 or 3,0 mg/ml or FCS at 5 10%. An evaluation of follicular development was conducted based on survival rate, antrum formation, increase in follicular diameter, oocyte viability and obtainment of fully-grown oocytes. It was observed that from the 12th cultivation day, the oercentage of surviving follicles under treatment with BSA at 3.0 mg/ml was greater than that of the other treatments (p< 0,05) when compared to those treated with BSAA on the last day of cultivation the mean diameter and antrum formation of follicles treated with BSA at 3,0 mg/ml were greater than those of follicles under other treatments (P < 0,05). With oocyte growth, the percentage of oocytes cultivated with BSA (3,0 mg/ml) thatwere destined for in vitro maturation (IVM: >110 diameter( was higher than that of other treatments. Moreover, under thistreatment, 86% of oocytes presented a germinal vesicle and 14% restarted meiosis, out of wich 3% were mature (metaphase II). In conclusion supplementing cultuvation medium with BSA at 3,0 mg/ml not only improves follicular development but also provides meiotically-competent oocytes after in vitro cultivation of caprine preantral isolated follicles.(AU)
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