Periódicos Brasileiros em Medicina Veterinária e Zootecnia

p. 437-441

Validation of the CellRox Deep Red ® fluorescent probe to oxidative stress assessment in equine spermatozoa

Lançoni, RenataArruda, Rubens Paes deAlves, Maíra Bianchi RodriguesOliveira, Letícia ZoccolaroSantos, Gabriel De Carli dosLemes, Kleber MenegonFlorez-Rodriguez, AndréaCeleghini, Eneiva Carla Carvalho

Considering the importance of ROS influence on sperm functionality and some limitations in sperm oxidative stress assessment methods, a field to studies of new techniques are still open. In this sense, the aim of this study is to validate the ROS detection technique through the CellRox Deep Red Reagent®probe in stallion sperm. Four stallions were used and the analyses were conducted on four replicates of semen samples from each of stallion (n = 16). The results of the polynomial regression presented a quadratic effect, high determination coefficient value (R2 = 0.88) and high significant P value (P < 0.0001). The CellRox Deep Red® fluorescent probe is able to detect reactive oxygen species in equine sperm, indicating accurately the occurrence of oxidative stress in stallion semen.(AU)

Texto completo