Eletroforese bidimensional e espectrometria de massa como ferramentas proteômicas aplicadas à definição de marcadores proteicos associados à eficiência reprodutiva de caprinos
Bezerra Júnior, Rosivaldo QuirinoMartins, Gabrielle RosemblitBarroso, Igor CiríacoMarinho, Rebeca CavalcanteAguiar, Tereza D'ávila de FreitasTeixeira, Maria Fátima da Silva
The growth and development of goat herds in the northeast are being observed due to the increase in livestock production in Brazil. This increase reflects demands of the consumer market for high quality product, which are obtained from flocks of high standard zootechnics. Current research illustrates the need for biomarkers that indicate an animal ́s reproductive potential, since this cannot be expressed solely with andrologic evaluation. For this reason, the expression of the proteins as biomarkers is a potential analysis. The proteins from the seminal plasma constituents are found in larger amounts in the form of associated complexes, playing a crucial role in all processes related to the fertilizing capacity of sperm. This analysis is performed by separation methods and simultaneous detection of proteins using techniques such as two-dimensional electrophoresis (2DE) or chromatography. These techniques are coupled with methods to identify and quantify expression levels of proteins by mass spectrometry that are increasingly efficient and sensitive. The aim of this review was to discuss the technique of two-dimensional electrophoresis combined with mass spectrometry as a tool in the analysis of protein expression within the field of proteomics.(AU)
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