Periódicos Brasileiros em Medicina Veterinária e Zootecnia

The effects of organic, inorganic, and nano-selenium on blood attributes in broiler chickens exposed to oxidative stress

Boostani, AlidadSadeghi, Ali AsgharNaser Mousavi, SeyedChamani, MohamadKashan, Nasser

Background: Physiological stress is one of many concerns facing the modern broiler producer. In this study, tert-butylhydroperoxide (tert-BHP) was used to induce oxidative stress. When birds exposed to stress, selenium is used in antioxidant enzymes to decrease the harmful effects of free radicals. In this regard, the source of selenium is important to provideenough protection. There are three forms of selenium as inorganic, organic and nano-selenium (Nano-Se). Recently, thelatter has attracted widespread attention because of large surface area, high surface activity, high catalytic efficiency, strongadsorbing ability and low toxicity. The present study aimed to compare different sources of selenium on blood attributesof male broilers subjected to oxidative stress.Materials, Methods & Results: Corn grain and soybean meal-base diets were formulated to meet the nutritional requirements of Cobb 500 broiler chickens. Three hundred twenty chicks were randomly assigned to receive diet without seleniumsupplementation (CON) or diets supplemented with 0.3 mg/kg nano-Se (NAN), organic Se (ORG), and inorganic Se (INO).The study was conducted as a completely randomized designed with a 2×4 factorial arrangement comprising four replicatesof 10 birds per each. Circulatory concentration of cholesterol and glucose were not influenced by the source of selenium.Experimental groups under the oxidative stress, however, recorded and increase in blood cholesterol and glucose. A higherheterophil to lymphocyte (H:L) ratio was found at oxidative condition. Furthermore, Se-supplemented groups were keptunder non-oxidative condition had a lower H:L ratio as compared to CON group, where the lower value was recorded forNAN birds (P < 0.05). The percentage of peripheral basophil in oxidatively-stressed experimental groups was significantlyhigher than their counterparts (P < 0.05)...(AU)

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