Periódicos Brasileiros em Medicina Veterinária e Zootecnia

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Occurrence, hemolytic toxins and antimicrobial resistance of Aeromonas hydrophila strains from dairy cow and anatolian water buffalo quarter milk samples in Turkey

Seker, EsraOzenc, ErhanKonak, SelahattinPamuk, SebnemKuyucuoglu, Yahya

Background: Motile aeromonads are considered to be one of the most important food-borne pathogens because of potential human health significance. Aerolysin and hemolysin are virulence factors playing a significant role in the pathogenesis of infections. Antimicrobial resistance is an important problem limiting therapeutic options. The main objective of the present study was to isolate motile Aeromonas species from cow and Anatolian buffalo quarter milk samples and determine the antimicrobial resistance and hemolysin (hlyA) and aerolysin (aerA) virulence genes of isolated species by PCR.Material, Methods & Results: The present study was carried out on apparently healthy 200 dairy cows and 103 Anatolian water buffaloes in different stages of lactation, hand-milked twice a day, held in private farms located in Afyonkarahisar province of Western Turkey. Before milking, 771 and 399 quarter milk samples were collected from cows and Anatolian buffaloes, respectively. For the isolation, APW, SAA and blood agar were used. The certain identification of isolates was made using API 20NE system. The strains were tested for susceptibility to 21 different antibiotics by disc diffusion test. Bacterial DNAs were extracted from all strains using a genomic DNA extraction kit and strains were screened for the presence of hlyA and aerA genes by PCR. While A. hydrophila was isolated from 22 (1.9%) of 1170 quarter milk samples, A. caviae and A. sobria were not detected in the examined milk samples. The isolation rate of A. hydrophila from cow and buffalo milk samples was 2.1% (n = 16) and 1.5% (n = 6), respectively. The highest resistance rate in strains was against ampicillin (100%), followed by ampicillin-sulbactam (95.5%), methicillin (95.5%) and cephazoline (81.8%). The most of strains were also resistant at least to one of the antibiotics.[...](AU)

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