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Periódicos Brasileiros em Medicina Veterinária e Zootecnia

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The c-jun N-terminal kinases (JNK) / mitogen-activated protein kinase (MAPK) is responsible for the protection of tanshinol (Danshensu) upon H2 O2 -induced L6 rat myoblast cell injury

Zhu, Dao-LiChen, Pei-Linan, Yuan

Background: Promoting L6 rat myoblast cell (L6 RMC) survival in the pro-apoptotic environment is critical to myoblast cell replacement for skeletal muscle degenerative disease therapy. Tanshinol (Danshensu), one of the principal bioactive components in salvia miltiorrhiza bunge, has been used widely in skeletal muscle system (SMS) diseases treatment and serves as an antioxidant to protect myoblast cells against oxidative stress. The present study was undertaken to investigate the protective effects of Tanshinol on L6 RMC injury induced by hydrogen peroxide (H2 O2 ). After challenge with 100 µM H2 O2 for 1h, loss of cell viability and excessive apoptotic cell death were observed in cultured L6 RMC, tanshinol treatment conferred protective effects against the loss of cellular viability in a concentration-dependent manner.Materials, Methods & Results: L6 rat myoblast cells (L6 RMC) were maintained DMEM containing 4.5 g/L glucose and supplemented with 10% (v/v) fetal bovine serum (FBS), 100 U/mL penicillin and 100 µg/mL streptomycin on tissue culture fl asks in a 37C humidifi ed atmosphere of 95% air and 5% CO2 . Cells were subcultured every 2-3 days. The cells were stained with SABC-Cy3 fl uorescence and measured by counting the nuclei. L6 RMC viability was determined by MTT assay in 96-well plates. The cells were counter-stained with H & E staining and Hoechst 33342 fl u

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