Periódicos Brasileiros em Medicina Veterinária e Zootecnia

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The age-related changes in SOD activity in bovine (Bos taurus) reproductive organs

Giergiel, MartaKankofer, Marta

Background: The appearance of reactive oxygen species (ROS) is connect with oxidative energy production particularly in reproductive organs. They characterize by increased metabolism, which may change during ageing. Their increase could be dangerous for germ cells. That is why the antioxidant protection is need. One of the main antioxidative enzyme is superoxide dismutase (SOD) which could be regulated by steroid hormones through genomic mechanism of action. But their level changed during ageing and maturation. However, there is still lack information about ageing alteration of SOD activity in bovine especially in reproductive organs.Materials, Methods & Results: The material for experiment consisted of reproductive organs: uterus (which was separated into endometrium and myometrium), both ovaries and corpus luteum collected immediately after slaughter at the slaughterhouse. Samples were taken from healthy cows aged between 16-24 months (n = 15) during luteal phase of cycle and female calves aged between 2 weeks and 2 months (n = 12) Total SOD activity was measured in homogenates of reproductive organs by use of the spectrophotometric method. PAGE electrophoresis was performed and Western blotting technique with specific anti-SOD antibodies as well as zymography confirmed the presence of enzymatic protein and the activity of SOD isoenzymes. The results of spectrophotometric

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