Sobrevivência de espermatozoides de catetos (Pecari tajacu) após congelação-descongelação no uso de diferentes diluentes
Campos, Lívia BatistaSilva, Mariana de Araújo daBezerra, José Artur BrilhanteCastelo, Thibério de SouzaPeixoto, Gislayne Christianne XavierSilva, Alexandre Rodrigues
Background: The development of semen cryopreservation protocols for collared peccaries (Pecari tajacu) would contribute to its preservation and multiplication. Nowadays, studies on this subject report the use of Tris and powdered coconutwater (ACP®-116c) as extenders. However, such studies are limited to the post-thawing immediate evaluation of samples.The knowledge on the sperm survival after thawing would be important in order to schedule the moment and the vial forartificial insemination. Therefore, the aim of the present research was to verify the effect of Tris and ACP extenders on thesperm survival of frozen-thawed semen, centrifuged or not, derived from collared peccaries.Materials, Methods & Results: Semen from 12 adult males collared peccaries were obtained by electroejaculation. Ejaculateswere immediately evaluated for sperm motility, vigor, viability, functional membrane integrity and morphology. Sampleswere diluted in Tris or ACP, both plus 10% egg yolk and 3% glycerol. Then, they were packaged into 0.25 mL straws, andstored in liquid nitrogen. After one week, samples were thawed at 38ºC/1 min and divided into two aliquots: one immediately diluted in the same extender, and the other submitted to centrifugation and then re-diluted. A thermorresistance testwas conducted including assessments at 0, 15, 30, 45, and 60 min as described for fresh semen. After analyzing the dataimmediately after thawing, we verify that coconut water provided a better preservation of sperm motility (41.3 ± 6.9%)and vigor (2.9 ± 0.2) than Tris, 33.1 ± 5.9% motile sperm with vigor 2.5 ± 0.2 (P < 0.05). During thermorresistance test...(AU)
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