Protein and lipid peroxidation intensity in cows and female calves
Giergiel, MartaZieliska, AnnaLegutko, KarolinaKankofer, Marta
Background: Reactive oxygen species alter cell membrane permeability and cause the damage to macromolecules due to peroxidative processes. The question was stated whether peroxidative processes are influenced by age and metabolic rate of particular tissue in cattle. The aim of current study was the investigation of age-related changes in lipid and protein peroxidation in chosen bovine tissues. The samples of liver, kidneys, heart and muscles were chosen, based on specifi c metabolic rate, to compare the differences between different types of tissues.Materials, Methods & Results: The samples of tissues were taken from healthy cows aged between 14 and 27 months (n = 13) and female calves aged between 2 weeks and 2 months (n = 12) immediately after slaughter. Tissues were homogenized, centrifuged and the supernatant was used for further determinations. The lipid peroxidation intensity was examined by the estimation of the content of intermediates (RC-DEPPD) and end products (TBARS) of this process. The protein peroxidative processes were measured by spectrophotometric (SH groups content) and spectrofluorimetric (Formylokinurenine and Bityrosine concentration) methods. Obtained results provided with the information that the intensity of peroxidative processes measured in present study differed significantly between cows and calves. Differences were related to metabolic...(AU)
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