Isolation of Plesiomonas shigelloides from infected cichlid fishes using 16S rRNA characterization and its control with probiotic Pseudomonas sp.
Girijakumari Nisha, RajagopalanRajathi, VellaikannuManikandan, RamarMarimuthu Prabhu, Narayanan
Background: Bacterial diseases are reported to cause heavy mortalities in both cultured and wild fishes throughout the world. In any field, diagnosis of disease plays a main role in curing the disease. In aquaculture, antibiotics are used to control the infectious diseases caused by bacteria. The use of antibiotics has been questioned as it experiences problems of bacterial resistance to antimicrobials and unacceptable residues in aquaculture products and environment. Recently, many studied have been performed in the application of probiotic as a control measure of infectious diseases. Therefore, this study has designed for the isolation of possible probiotic bacteria which inhibit the growth of specific pathogenic bacteria isolated from a fresh water ornamental fish.Materials, Methods & Results: Diseased cichlid fishes were brought to the laboratory for the isolation of causative bacteria. A total of three strains were isolated (APG01, APG02 and FA3) from the serially diluted samples of gut, gill and spleen. The isolated pathogenic bacteria were injected at the concentration of 105 cfu/mL to healthy cichlid fishes to find out its virulency in vivo at varying time duration (12, 24, 36 and 48 h) of exposure for 15 days. Among the three isolates, only APG01 caused 100% mortality of cichlid fishes at 48 h of exposure. APG01 was subjected to 16S rRNA characterization and confirTexto completo