Periódicos Brasileiros em Medicina Veterinária e Zootecnia

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Enhancement of immune responses against iranian isolate of FMD-type O/IRN/1/2010 based on VP1 and human hsp70 genes and comparison with conventional vaccine

rahnazrahnazrahnazrahnazYazdanpanah, SolmazSoleimanjahi, HooriehMahravani, HoomayonShafaee, KamalodinHadi Razavi, MohammadRezaee, Abass

Background: Foot and mouth disease (FMD) is the causative agent and one of the most transmissible livestock diseases which cause important economic losses. The genome of FMD virus is a positive-sense, single-stranded RNA and it wrapped with 60 copies of 4 structural proteins, VP1, VP2, VP3 and VP4. The VP1 is a major immunogenic antigen with critical epitopes for inducing immune responses. The current vaccine, which successfully prevents disease, includes inactivated whole virus antigen. However, it is not without problems. The aim of this study is enhancement of immune responses against Iranian isolate of FMD-type O/IRN/1/2010 based on VP1 and human HSP70 fusion protein in BALB/c mice.Materials, Methods & Results: In this study FMD virus type O/IRN/2010 was isolated from vesicles of the infected cattle in Qoum, and serotyped as a new linage of type O (PanAsia-linage 2) in Iran. The isolated FMD virus was propagated on IBRS2 cell line and whole RNA of the infected cells was extracted by commercial kit instruction. The extracted RNA was amplifi ed using VP1 gene-specifi c primer pairs by means of one-step RT-PCR. The specifi c primer pair was designed by AllelID6 software. There are sequences of Kpn I and BamH I restriction enzymes and three overhanging nucleotides at the start of forward and reverse primers, respectively. The VP1 nucleotide sequence was deposit in Genbank-N

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