Periódicos Brasileiros em Medicina Veterinária e Zootecnia

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Biofilm production and antibiotic susceptibility of planktonic and biofilm bacteria of canine dental tartar isolates

Manuel, AtulyaVenkata Rao, JosyulaJohn, KoshyMathew Aranjani, Jesil

Background: In nature, bacteria prefer to live as a community rather than planktonic cells. Biofi lm is such a community of bacteria embedded in a coherent cluster of polysaccharides, proteins and nucleic acids. The biofi lm formation in the dental cavity can result in the development of periodontal diseases. Biofi lm in the oral cavity can affect the general health of the dog and the bacterial toxins can damage the visceral organs engaged in detoxifi cation. It can also be detrimental to human health when transferred via animal bites. Aim of this study is to isolate the organisms from the dental tartars of dogs and to study their response to antibiotics in both planktonic and biofi lm mode of life.Materials, Methods & Results: Organisms were isolated from fourteen canines with dental tartar using brain heart infusion agar medium. They were identifi ed as per Bergys Manual. Antibiogram of the isolates were done with the Kirby Bauer method using standard antibiotic discs. Initial screening of biofi lm production was done using the congo red agar assay. Quantifi cation of biofi lms were done using a standard microtitre plate method using crystal violet staining. The minimum inhibitory concentration and minimum biofi lm eradication concentration were compared to determine the change in the antimicrobial susceptibility pattern of dental tartar isolates when they go from the pl

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