Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of rainbow trout (Oncorhynchus mykiss) seminal plasma proteins and their correlation with semen characteristics
Asadpour, RezaTayefi-Nasrabadi, HosseinSheikhzadeh, Najmeh
Background: Fish seminal plasma is a complex mixture made of different components. In few studies on some fish species such as rainbow trout, Nile tilapia and jundiá fish (Rhamdia quelen) characterization of seminal plasma proteins were shown. Meanwhile there is little information regarding the correlation between specific seminal proteins and semen characteristics in rainbow trout. The objective of the present study was to evaluate the seminal plasma protein profiles and their relation with some semen characteristics in rainbow trout. Materials, Methods & Results: Nine mature male rainbow trout with total weight 2300 ± 200 g were used. A total of nine male fish were anaesthetized by clove oil bath (50 µl L-1) and semen was collected by abdominal massage. Semen variables (spermatocrit, concentration, duration of activity, percent of motility, total protein) were recorded. Seminal plasma was also separated by centrifugation and subjected to SDS-PAGE analysis. Gel images were analysed to determine molecular weight and relative protein content (pixel density) using the Total Lab TL120 computer program. Spermatocrit (%), sperm concentration (cell mL-1), duration of sperm activity (sec), percentage of sperm motility and total protein (mg dL-1) were 22.67, 12.35 × 109, 36.56, 89.33 and 81.49 respectively. Nine protein bands were identified on the 15% gel ranging from 11.74 to 68.
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