Periódicos Brasileiros em Medicina Veterinária e Zootecnia

p. 01-06

Low water temperature retards white spot syndrome virus replication in Astacus leptodactylus crayfish

Heidarieh, MarziehSoltani, MehdiMotamedi Sedeh, FarahnazSheikhzadeh, Najmeh

Background: White spot syndrome virus is a pathogen of major economic importance to cultured penaeid shrimp industries globally. White spot disease can cause mortalities reaching 100% within 3-10 days of gross signs appearing. WSSV replicates in tissues from mesoderm and ectoderm embryonic origin and characteristically induces cell nuclei hypertrophy and intra nuclear inclusion bodies. WSSV also has an extremely broad host range including marine and freshwater crabs and crayfishes, copepods and other arthropods in addition to shrimp. Water temperature can affect the progress of WSD in crustaceans but there have been conflicting reports of higher temperatures protecting Litopenaeus vannamei shrimp but lower temperatures protecting Marsupenaeus japonicas. Here we have examined how 2 water temperatures affect the progression of WSD in the freshwater crayfish Astacus leptodactylus. Materials, Methods & Results: Freshwater Astacus leptodactylus crayfi sh (20 ± 0.5 g) were obtained from Aras dam, Iran. Crayfish were acclimated for 10 days in an aerated indoor cement tank with flow-through of dechlorinated freshwater with the flow rate set at 0.5 L/s, water temperature 15 ± 1ºC and dissolved oxygen 5.2 ppm. Two groups of 25 crayfish were allocated to tanks being supplied 15 ± 1C water and 2 groups of 25 crayfish were allocated to tanks being supplied 25 ± 1C water. Each crayfish

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