Periódicos Brasileiros em Medicina Veterinária e Zootecnia

p. 01-05

Cell localization of ACTH, dopamine, and GnRH receptors and PPARy in bovine corpora lutea during diestrus

Parillo, FrancescoCatone, GiuseppeGobbetti, AnnaZerani, Massimo

Background: Corpora lutea (CL) are transient organs, essentials for the pregnancy to be successful, that results from the ovulatory follicle rupture. If pregnancy fails to occur, the CL undergo luteolysis, a dynamic regression process that ends with their complete functional and structural demise. The life span of CL is characterized by luteal development, maintenance, and regression regulated by complex interactions between luteotropic and luteolytic mediators. The life-span of bovine CL is regulated by a multifactorial system that includes various hormonal regulators that up to date are not yet well studied, therefore the aim of the present study was to evaluate the immunohistochemical presence of receptors for adrenocorticotropic hormone (melanocortin-2 receptor, MC2R), dopamine (DR1-5), gonadotropin-releasing hormone (GnRHR), and peroxisome proliferators-activated receptor (PPAR) in early, mid, late and regressive CL during diestrous cycle of bovine. Materials, Methods & Results: Ovaries from 24 clinically healthy cyclic cows were collected from licensed abattoirs. The animals had undergone a general veterinary assessment of their reproductive tract and ovaries before slaughter to exclude any apparent abnormalities and early pregnancy. Corpora lutea were separated by blunt dissection from the surrounding ovarian tissues and classified into four groups, covering the e

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