Periódicos Brasileiros em Medicina Veterinária e Zootecnia

p. 01-08

Relationship between mineral composition of seminal plasma and semen quality in various ram breeds

Asadpour, Reza

Background: Seminal plasma of mammals is a complex fluid, which serves as a carrier for the spermatozoa on their journey from the male testes to their target, the oocyte. Seminal plasma contains a variety of biochemical components, some of which are relatively specific for the regulation of sperm function. The objective of this study was to investigate enzyme activities aspartate-amino-transferase (AST), alkaline phosphatase (ALP), lactate-dehydrogenase (LDH) and concentrations of macroelements[ sodium (Na+ ) and potassium (K+ ) and calcium (Ca2+) ] in seminal plasma. Moreover the present study was performed to investigate proteins in ram seminal plasma and the correlation between specific proteins and semen characteristics in cross breed ram.Materials, Methods & Results: Sixteen crossbred fertile rams (four Baluchi × Moghani, four Ghezel × Baluchi, four Ghezel × Merino, and four Merino × Moghani) were used in this study. Three ejaculates of each animal were collected during the breeding season and examined spermatozoa with light microscopy for motility, concentration, for dead sperm and morphology. Seminal plasma was harvested by centrifugation and then subjected to analysis enzyme, mineral composition and SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Total protein, AST, ALP, LDH, Na+ , K+ and calcium Ca2+ were measured. Spearmans correlation coefficient test was app

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