Periódicos Brasileiros em Medicina Veterinária e Zootecnia

p. 01-04

Repeated collection of cerebrospinal fluid from the lumbosacral region of ewes

Guillermo Quintela, HomeroBiell, AlejandroTorterolo, PabloLagos, PatriciaAlzugaray, StefaníaUngerfeld, Rodolfo

Background: Cerebrospinal fl uid is a vital fl uid from the central nervous system. Since CSF contains proteins, enzymes, hormones, neuropeptides and neurotransmitters that play critical regulatory roles in many different physiological processes, it has been extensively studied to explore different nervous disorders. Since CSF is a vital fl uid from the CNS, used for clinical examination of the CNS in ruminants and other domestic species. CSF may be collected in ewes under field conditions, which allows the diagnosis of bacterial and metabolic diseases, as well as using it for cytological studies and biochemical analysis. Depending on the study, in opportunity the sampling protocol should be repeated to measure dynamic changes in the parameters selected for the analysis. Under fi eld conditions, obtaining CSF samples from ewes is a diffi cult task. Thus, the aim of this work was to determine if it is possible to obtain repeated extractions of CSF by lumbosacral puncture from the same ewes under fi eld conditions.Materials, Methods & Results: The CSF was sampled in three successive weekly collections from nine ewes sedated with ketamine. The procedure collections were made by the same trained operator, who stood behind the ewe, facing its back. Having checked that the sagital plane of the animal was perpendicular to the horizontal plane the puncture point was found by manual

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