Trypanosoma evansi: ultrastructural cardiac muscle and cardiac microvasculature changes in experimental murine infections
Tejero, FelixLorena Arias-Mota, LourdesRoschman-González, AntonioMaría Aso, PedroJosé Finol, Héctor
Background: : : : Trypanosoma evansi is the etiologic agent of the equine trypanosomosis, a disease related to the detriment of the extensive bovine farming in the Venezuelan grasslands. Even though macroscopic pathologies such as anemia, pale mucosa, icteric tissues, generalized edema, splenomegaly, liver and renal hypertrophy, abortion, anoestrus, emaciation, lymphadenopathies, striated muscle atrophy as well as epicardiac and endocardiac hemorrhages have been describedfor infections with the agent, no reports of any heart ultrastructural change in experimental or natural infections induced by Venezuelan T.evansi isolates are available. This, a transmission electron microscopic approach to the problem was needed. This work describes cell features of the cardiac myocyte and the cardiac microvasculature ultrastructure in mice experimentally infected with an equine local isolate of T. evansi, also providing an account of the infection with the mices survival. Material, Methods & Results: NMRI Mus musculus were inoculated with a Venezuelan T. evansi isolate derived from a naturally infected Equus caballus. From day three post-infection, and every other day until the mices death, one rodent was randomly sacrificed, the heart apex was isosmotically removed and cut in symmetrical blocks, which were fixed, post-fixed, dehydrated, infiltrated, included, sectioned, contrasted
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