Effects of oxidative stress on liver, brain and spinal cord of rats using L-NAME and treated with hydroxyurea: a model of sickle cell complication

Santos Neto, Abilio Torres dosSilva, Iandara SchettertIvo, Maria LuciaRodrigues, Camila TozakiParisotto, Eduardo BenedettiRamalho, Rondon TostaMonteiro, Geanlucas Mendes

Purpose: To analyze the serum levels of nitric oxide and correlate them with the levels of thiobarbituric acid reactive substances (TBARS) in liver, brain and spinal cord of animals using L-NAME and treated with hydroxyurea. Methods: Eighteen male albino Wistar rats were divided into three groups. NG-nitro-L-arginine methyl ester (L-NAME) was intraperitoneally administered to induce oxidative stress. TBARS and plasma nitric oxide levels were analyzed in all groups. Histopathology of the liver and vascular tissue was performed. Results: Statistically significant differences were seen in liver, brain and spinal cord TBARS levels. Conclusions: Following the use of L-NAME, hepatic tissue increased the number of Kupffer cells as oxidative stress and inflammatory response increased. The use of L-NAME caused an increase in lipid peroxidation products and, consequently, in oxidative stress in animals. Hydroxyurea doses of 35 mg / kg / day reduced TBARS values in liver, brain and spinal cord.(AU)

1. NG-nitroarginine methyl ester
2. Rats
3. Oxidative stress
4. Hydroxyurea
5. Sickle cell
6. Anemia