Effects of baicalin on inflammatory reaction, oxidative stress and PKDl and NF-kB protein expressions in rats with severe acute pancreatitis

Qian, YunzhongChen, YiWang, LinyangTou, Jinfa

Purpose: To investigate the effects of baicalin on inflammatory reaction, oxidative stress and protein kinase D1 (PKD1) and nuclear factor-kappa B (NF-B) protein expressions in severe acute pancreatitis (SAP) rats. Methods: Sixty rats were divided into sham operation, model, and low-, medium- and high-dose baicalin group. SAP model was established in later 4 groups. The later 3 groups were injected with 0.1, 0.2 and 0.4 ml/100 g 5% baicalin injection, respectively. At 12 h, the serum SAP related indexes and inflammatory factors, peripheral blood CD3 and T cell percentages, wet/dry ratio and pancreas ascites volume, oxidative stress indexes and PKD1 and NF-B protein expressions in pancreatic tissue were determined. Results: Compared with model group, in high-dose baicalin group the wet/dry ratio and ascites volume, serum amylase level, phospholipase A2 activity, TNF-, IL-1 and IL-6 levels, and pancreatic malondialdehyde level and PKD1 and NF-B protein expression were significantly decreased (P 0.05), and peripheral blood CD3 and T cell percentages and pancreatic superoxide dismutase and glutathione peroxidase levels were significantly increased (P 0.05). Conclusion: Baicalin can resist the inflammatory reaction and oxidative stress, and down-regulate protein kinase D1 and nuclear factor-kappa B protein expressions, thus exerting the protective effects on severe acute pancreatitis in rats.(AU)

1. Rats
2. Pancreatitis
3. NF-kappa B
4. Protein Kinases
5. Oxidative Stress