Effects of the essential oil of Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm. on healing and tissue repair after partial Achilles tenotomy in rats
Santos-Júnior, LucianoOliveira, Tassia Virgínia de CarvalhoCândido, Janaína FariasSantana, Danielle Souza dePereira Filho, Rose NelyPereyra, Beatriz Benny SungailaGomes, Margarete ZanardoLima, Sônia OliveiraAlbuquerque-Júnior, Ricardo Luiz Cavalcanti deCândido, Edna Aragão Farias
Purpose: To investigate the cellular response to injury, analyzing histopathologic changes associated with increased cellularity, degeneration and disorganization of collagen fibers. Methods: Thirty wistar rats were divided in two groups after partial Achilles tenotomy: the right hind paw were treated with the essential oil of Alpinia zerumbet (EOAz), diluted to 33% (0.3 mL kg-1), and the left hind paw received sunflower oil for 3, 14, 30 and 90 days. Statistical significance was determined using a Chi-square and Pearson Correlation qualitative variables test. Moreover, Mann-Whitney U-test test for comparison between different groups of the same cell, one-way ANOVA, and Tukeys test of quantitative measurement. Results: A decrease hyperemia (p 0.001) was observed in the acute phase of inflammatory cell number (p 0.001), whereas sub-acute phase was marked by significant correlation with macrophages in fibroblasts (r = 0.17, p = 0.03), with probable induction a dense and modeled tissue. At chronic phase, it was found an increase in the number of fibroblasts and a higher percentage of type I collagen fibers (78%) compared with control collagen fibers (55%). Conclusion: Oil of Alpinia zerumbet stimulated the process of maturation, organization and tissue repair which gave it greater resistance.(AU)
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