Periódicos Brasileiros em Medicina Veterinária e Zootecnia

p. 264-270

Antioxidant effect of Legalon(r) SIL in ischemia-reperfusion injury of rat skeletal muscle

Ergün, YusufÜremiş, MuhammedKılınç, MetinAlıcı, Tuğrul

PURPOSE:To evaluated the potential antioxidant agent Legalon(r)SIL (silibinin-C-2',3-bis(hydrogensuccinat)) in the skeletal muscle of rats.METHODS:IRI was achieved via tourniquet application in Wistar-albino rats. Experimental groups were chosen as (i) sham control, (ii) IRI (3+2 h), (iii) IRI and Legalon(r)SIL-50 (50 mg/kg/i.p.), (iv) IRI and Legalon(r)SIL-100 (100 mg/kg/i.p.), and (v) IRI and Legalon(r)SIL-200 (200 mg/kg/ i.p.). Muscle viability (evaluated by triphenyltetrazolium chloride dye method), malondialdehyde, superoxide dismutase, catalase, and glutathione peroxidase were assessed in muscle samples using a spectrophotometer.RESULTS:Although viability of the injured limb non-significantly declined in the IRI group, administration of Legalon(r)SIL did not prevent injury. However, dramatic increase observed in malondialdehyde levels in the IRI group was prohibited by Legalon(r)SIL in a statistically significant manner. In comparison with the sham-control group, IRI and Legalon(r)SIL administration did not cause any significant alterations in the levels of superoxide dismutase, catalase, and glutathione peroxidase.CONCLUSION:Although Legalon(r)SIL was not sufficient to prevent muscle injury in terms of viability, it is found to be an effective option to reduce reactive oxygen species-induced cell injury.(AU)

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